19 research outputs found
Cytologic characteristics of adenoid cystic carcinoma of the cervix uteri: case report [CitoloÅ”ke karakteristike adenoid cistiÄnog karcinoma vrata maternice: prikaz sluÄajeva]
Adenoid cystic carcinoma is a rare malignancy, usually found in the salivary glands, although this unusual tumor can be found at other sites of the body. In particular, regarding adenoid cystic carcinoma of the cervix (ACCC) most frequently reports are given for postmenopausal women. In this respect, our work is one among very few in the literature that considers a cytologic picture of this uncommon cervix carcinoma. The case of 74 year old patient with postmenopausal bleeding is described. Both Pap smear and air dried smear of the uterine cervix were analyzed. The cytologic findings revealed very few small clusters of abnormal glandular cells, as well as some amorphous eosinophilic globule-like material, with granulomatous and necrotic background. The latter includes a lot of histiocytes, multinucleated giant cells, large aggregates of epitheloid cells and lymphocytes. Histology revealed the diagnosis of ACCC. We emphasize the importance of careful screening of Pap smear that might be crucial in the case of suspicious clinical findings in postmenopausal women, when the possibility of ACCC has to be considered
Influence of the surface roughness of coated and uncoated papers on the digital print mottle
Many factors influence the occurrence of print mottle in prints. In printing process three main components are involved: printing press, substrate and toner. They can be considered as separate components, but in most cases their interaction influences the quality of the print. The goal of this work was to examine the influence of surface roughness of different types of paper (coated and uncoated on print mottle of electrophotographic digital prints. We set up a hypothesis that print mottle will be more apparent on rougher surfaces. In the experimental part we printed four different substrates with different surface properties on electrophotographic printing press. Morphology of the papers surface was analysed using atomic force microscopy (AFM) from which surface properties were calculated. For print mottle characterization Gray level co-occurrence matrix (GLCM) method was used. Based on the measurements and results we can conclude, contrary to the initial hypothesis, that uncoated papers with rougher surfaces produce smaller print mottle values
AtipiÄne glandularne stanice u papa testu kao primarna prezentacija seroznog borderline tumora jajnika
The Pap test is designed as a screening test to detect primarily carcinoma of the cervix and its precursors, most often of squamous type. Rarely atypical or malignant cells found in cervical smear can be indication for the existence of an extrauterine disease. We present a case of a 26 year old woman with very small asymptomatic ovarian bilateral borderline tumors whose diagnosis was triggered by finding of atypical glandular cells with psammoma bodies on Pap smear. She had a history of abnormal Pap smears and was admitted to our Hospital for colposcopy. Pap smear was taken and reported as atypical glandular cells (AGC) with psammoma bodies. Colposcopically directed biopsy along with an endocervical curettage showed no abnormalities as well as transvaginal ultrasound examination. Three months later control Pap smear confirmed AGC with psammoma bodies (suggestive of endometrial or serous origin), ultrasound showed the presence of indistinctive, hyperechoic mass 2 cm in diameter in the left ovary along with slightly elevated CA-125 and diagnostic hysteroscopy and laparoscopy was performed. At laparascopy, both ovaries had surface cauliflower like papillary projections measuring up to 3 cm. A histological examination revealed bilateral serous borderline tumor.Papa test je test probira primarno namijenjen za rano otkrivanje karcinoma cerviksa i njegovih prekursora, najÄeÅ”Äe ploÄastog tipa. Rijetko nalaz atipiÄnih ili malignih stanica u Papa testu može biti prvi znak tumora ekstrauterinog porijekla. U ovom radu prikazujemo sluÄaj 26 godiÅ”nje pacijentice s vrlo malim asimptomatskim bilateralnim borderline tumorima jajnika Äija je dijagnoza pokrenuta nalazom atipiÄnih žljezdanih stanica sa psamomskim tjeleÅ”cima u Papa testu. Pacijentica je upuÄena u naÅ”u bolnicu na kolposkopiju radi abnormalnih nalaza Papa testa. Kod nas uzeti Papa test pokazao je atipiÄne glandularne stanice (AGC) sa psamomskim tjeleÅ”cima. Kolposkopski voÄena biopsija i endocervikalni ekskohleat nisu pokazali abnormalnosti, kao ni transvaginalni ultrazvuÄni pregled. Tri mjeseca kasnije ponovljeni Papa test potvrÄuje AGC sa psamomskim tjeleÅ”cima (porijeklo endometralno ili serozno), ultrazvuÄni nalaz opisuje uz lijevi jajnik nejasnu hiperehogenu tvorbu promjera 2 cm, uz blago poviÅ”eni CA 125 zbog Äega je uÄinjena dijagnostiÄka histeroskopija i laparoskopija. Na operaciji su naÄeni jajnici obostrano s tumorskim tvorbama karfiolastog izgleda veliÄine do 3 cm. PatohistoloÅ”ki je potvrÄen serozni borderline tumor oba jajnika
Liquid-based cytology - new possibilities in the diagnosis of cervical lesions [TekuÄa citologija - nove moguÄnosti u dijagnostici lezija vrata maternice]
Liquid-based cytology (LBC) enables the use of supplementary methods in the diagnosis and prognosis of cervical lesions. The aim of this study was to analyze the correlation between p16INK4a immunoexpression in ThinPrep cervical cytologic samples and human papillomavirus (HPV) detection by polymerase chain reaction (PCR) from the same sample. LBC-ThinPrep (Cytyc, USA) cervical cytology samples, prepared and stained by Papanicolaou method, were analyzed using modified Bethesda cytologic classification named Ā»Zagreb 2002Ā«. A second ThinPrep slide, prepared from the same sample, was immunostained for p16INK4a using CINtec p16INK4a Cytology Kit (DakoCytomation, Denmark). Increased expression of the high-risk (HR) HPV E6 and E7 oncogenes results in a highly specific increase in p16 protein expression and overexpression of p16INK4a acts as a potential biomarker for cervical cancer progression from premalignant lesions. Brown nuclear and/or cytoplasmic staining of abnormal cells was considered a positive result. Residual material was used for 13 HR HPV-DNA detection by the PCR based AMPLICOR HPV test (Roche Molecular Systems). A total of 120 ThinPrep Pap tests with the following cytologic diagnoses: 17 within normal limits, 17 atypical squamous cell (ASC) (7 ASC of undetermined significance /ASCUS/ and 10 ASC of high-grade squamous intraepithelial lesions cannot be excluded /ASC-H/), 26 low-grade squamous intraepithelial lesions (LSIL) corresponding cervical intraepithelial neoplasia (CIN) I, 57 high-grade SIL (HSIL) i.e. 24 CIN II and 33 CIN III and 3 squamous cell carcinoma (SCC) were included in the study. All CIN III (n=33) and SCC (n=3) specimens expressed p16INK4a immunoreactivity, whereas the HR HPV test was positive in 97% (32/33) of CIN III and 100% (3/3) of SCC specimens. The p16INK4a biomarker was positive in 87.5% (21/24) of CIN II and 69% (18/26) of CIN I, while the HR HPV was positive in 75% (18/24) of CIN II and 50% (13/26) of CIN I. In ASCUS cytology, p16INK4a and HR HPV showed the same rate of positivity (28.5%; 2/7). Expression of p16INK4a was detected in all cytologic (10/10) ASC-H lesions, in contrast to HR HPV detected in only 20% (2/10) of ASC-H cases. These data suggest the p16INK4a evaluation in ThinPrep cervical samples to be significantly associated with HR HPV testing by PCR in the same sample for the diagnosis of HSIL lesions and cervical carcinomas. A prospective study with longer follow up may clarify the predictive values in the management of LSIL and ASC diagnosis
Cytologic Characteristics of Adenoid Cystic Carcinoma of the Cervix Uteri ā Case Report
Adenoid cystic carcinoma is a rare malignancy, usually found in the salivary glands, although this unusual tumor can be found at other sites of the body. In particular, regarding adenoid cystic carcinoma of the cervix (ACCC) most frequently reports are given for postmenopausal women. In this respect, our work is one among very few in the literature that considers a cytologic picture of this uncommon cervix carcinoma. The case of 74 year old patient with postmenopausal bleeding is described. Both Pap smear and air dried smear of the uterine cervix were analyzed. The cytologic findings revealed very few small clusters of abnormal glandular cells, as well as some amorphous eosinophilic globule-like material, with granulomatous and necrotic background. The latter includes a lot of histiocytes, multinucleated giant cells, large aggregates of epitheloid cells and lymphocytes. Histology revealed the diagnosis of ACCC. We emphasize the importance of careful screening of Pap smear that might be crucial in the case of suspicious clinical findings in postmenopausal women, when the possibility of ACCC has to be considered
Cytologic Characteristics of Adenoid Cystic Carcinoma of the Cervix Uteri ā Case Report
Adenoid cystic carcinoma is a rare malignancy, usually found in the salivary glands, although this unusual tumor can be found at other sites of the body. In particular, regarding adenoid cystic carcinoma of the cervix (ACCC) most frequently reports are given for postmenopausal women. In this respect, our work is one among very few in the literature that considers a cytologic picture of this uncommon cervix carcinoma. The case of 74 year old patient with postmenopausal bleeding is described. Both Pap smear and air dried smear of the uterine cervix were analyzed. The cytologic findings revealed very few small clusters of abnormal glandular cells, as well as some amorphous eosinophilic globule-like material, with granulomatous and necrotic background. The latter includes a lot of histiocytes, multinucleated giant cells, large aggregates of epitheloid cells and lymphocytes. Histology revealed the diagnosis of ACCC. We emphasize the importance of careful screening of Pap smear that might be crucial in the case of suspicious clinical findings in postmenopausal women, when the possibility of ACCC has to be considered
Digital morphometry of cytologic aspirate endometrial samples [Digitalna morfometrijska analiza citoloŔkih uzoraka aspirata endometrija]
Unlike cervical cytology, morphological cytology criteria in the differential diagnosis of endometrium have not yet been clearly defined, and methods to allow for more precise evaluation of endometrium status have been searched for. The aim of the present study was to assess the value of morphometric nucleus analysis of cytologic aspirate endometrial samples in proliferative, hyperplastic and malignant endometrium by use of digital image analysis. Morphometric analysis was performed on archival cytologic aspirate endometrial samples (at least 10 per group) stained according to Papanicolaou (n=77) and May-GrĆ¼nwald-Giemsa (MGG; n=80) with the following histopathologic diagnoses: proliferative endometrium, hyperplasia simplex, hyperplasia complex, hyperplasia complex atypica, and adenocarcinoma endometriodes endometrii (grade I, II and III). Interactive image analysis (nuclear area, convex area, perimeter, maximum and minimum radius, length and breadth, as well as nucleus form factor and elongation factor) was performed by use of the SFORM software (VAMSTEC, Zagreb) on at least 50 (Papanicolaou stain) and 100 (MGG stain) well preserved endometrial epithelial cell nuclei without overlapping, at magnification of Ā“1000. Statistical data analysis was done by use of the Statistica Ver. 6 statistical package. Multivariate analysis (ANOVA) distinguished malignant, hyperplastic and proliferative endometrium according to all morphometric variables with both staining methods (p0.05) from atypical hyperplasia, adenocarcinoma and proliferative endometrium only according to the nucleus form factor and elongation factor (Papanicolaou stain), whereas malignant and atypical hyperplastic endometrium (MGG stain) differed statistically significantly (p0.05). According to the cytologic staining method, morphometric parameters were considerably higher in MGG stained endometrial samples, reaching the level of statistical significance (p0.05) in the groups of hyperplasia simplex and complex, well differentiated adenocarcinoma (form factor) and atypical hyperplasia (elongation factor). A combination of cytomorphology and the morphometric variables assessed in this study can yield useful information on the cytologic state of endometrium, with special reference to the possible differentiation of the group of hyperplasia without atypia from the group of adenocarcinoma and atypical hyperplasia
Uloga citologije u otkrivanju preinvazivnih i invazivnih promjena stidnice ovisno o naÄinu uzimanja uzorka
Eksfolijativna citologija stidnice kao neinvazivna metoda koristi se u dijagnostici i praÄenju promjena na stidnici, osobito onih kod kojih nije indicirana biopsija. Dosad se tradicionalno obrisak uzimao Å”patulom te su citoloÅ”ki uzorci Äesto bili slabo celularni. Kako bi se poboljÅ”ala celularnost uveden je postupak uzimanja endocervikalnom Äetkicom.
U retrospektivnoj studiji obuhvaÄeno je 1866 uzoraka sa stidnice uzetih u razdoblju od 1. srpnja 2011. do 31. prosinca 2013. Analizirana je primjerenost uzoraka kao i omjer nalaza atipiÄnih ploÄastih stanica (ASC) prema nalazu ploÄaste intraepitelne lezije ili karcinoma (SIL+), ovisno o ambulanti u kojoj je uzorak uzet, te citohistoloÅ”ka korelacija.
U onkoloÅ”koj ambulanti specijaliziranoj za bolesti stidnice u kojoj je veÄina uzoraka uzimana Äetkicom bilo je najmanje neprimjerenih uzoraka zbog oskudnosti (3,4%), kao i zadovoljavajuÄih, ali oskudnih uzoraka (5,6%), a najviÅ”e ovakvih uzoraka bilo je u ginekoloÅ”kim ambulantama primarne zdravstvene zaÅ”tite (13,7% odnosno 25,3%) u kojima su obrisci uglavnom uzimani na tradicionalan naÄin ā Å”patulom. Usporedili smo omjer ASC:SIL+ nalaza kod potpuno zadovoljavajuÄih nalaza ovisno o ambulanti u kojoj su uzeti uzorci: u ambulanti specijaliziranoj za bolesti stidnice bio je 2,6:1, u ostalim onkoloÅ”kim ambulantama 1,9:1, u ostalim ambulantama klinike 3,5:1, dok je u ambulantama primarne ginekologije bio 2,3:1. Kod zadovoljavajuÄih, ali oskudnih nalaza ovaj omjer ASC:SIL+ varirao je od 5:1 do 11:0 ovisno o ambulanti u kojoj su uzorci uzeti. CitohistoloÅ”ka korelacija 159 sluÄajeva pokazala je visoku osjetljivost u otkrivanju preinvazivnih (94,1%) i invazivnih (100,0%) promjena, ali nisku specifiÄnost (35,4%).
Uzorci sa stidnice uzeti endocervikalnom Äetkicom znaÄajno su celularniji od uzoraka uzetih Å”patulom i time bolji za citoloÅ”ku analizu te je manji omjer ASC:SIL+ nalaza. CitoloÅ”ka analiza uzoraka sa stidnice ne može zamijeniti biopsiju, ali kod kliniÄki nejasnih lezija može pomoÄi u postavljanju indikacije za daljnji postupak, kao i u praÄenju bolesnica s potvrÄenom bolesti
Influence of Paper Properties and Printing Machines Properties on Optical Density in Electrophotography
Electrophotography is affected by several parameters, including printing machines and paper type, which determine the final quality of reproduction. For the
investigation of those effects, tests were done on the three types of electrophotography printing machines and eight types of uncoated paper with different properties of
weight, surface roughness, whiteness, brightness and opacity. Samples were printed with cyan, magenta, yellow and black toner under the standard, identical conditions.
The assessment of standard print quality parameter (optical density) was done using spectrodensitometer Techkon Spectro Dens. At the same time, the paper substrates
were characterized using the atomic force microscopy (AFM) as well as by spectrophotometric measurements (X Rite Eye-One, i1). Obtained results showed that the
reproducibility significantly depends on the type of printing machine rather than optical and mechanical properties of paper
Liquid-Based Cytology ā New Possibilities in the Diagnosis of Cervical Lesions
Liquid-based cytology (LBC) enables the use of supplementary methods in the diagnosis and prognosis of cervical lesions. The aim of this study was to analyze the correlation between p16INK4a immunoexpression in ThinPrep cervical cytologic samples and human papillomavirus (HPV) detection by polymerase chain reaction (PCR) from the same sample. LBC-ThinPrep (Cytyc, USA) cervical cytology samples, prepared and stained by Papanicolaou method, were analyzed using modified Bethesda cytologic classification named Ā»Zagreb 2002Ā«. A second ThinPrep slide, prepared from the same sample, was immunostained for p16INK4a using CINtec p16INK4a Cytology Kit (DakoCytomation, Denmark). Increased expression of the high-risk (HR) HPV E6 and E7 oncogenes results in a highly specific increase in p16 protein expression and overexpression of p16INK4a acts as a potential biomarker for cervical cancer progression from premalignant lesions. Brown nuclear and/or cytoplasmic staining of abnormal cells was considered a positive result. Residual material was used for 13 HR HPV-DNA detection by the PCR based AMPLICOR HPV test (Roche Molecular Systems). A total of 120 ThinPrep Pap tests with the following cytologic diagnoses: 17 within normal limits, 17 atypical squamous cell (ASC) (7 ASC of undetermined significance /ASCUS/ and 10 ASC of high-grade squamous intraepithelial lesions cannot be excluded /ASC-H/), 26 low-grade squamous intraepithelial lesions (LSIL) corresponding cervical intraepithelial neoplasia (CIN) I, 57 high-grade SIL (HSIL) i.e. 24 CIN II and 33 CIN III and 3 squamous cell carcinoma (SCC) were included in the study. All CIN III (n=33) and SCC (n=3) specimens expressed p16INK4a immunoreactivity, whereas the HR HPV test was positive in 97% (32/33) of CIN III and 100% (3/3) of SCC specimens. The p16INK4a biomarker was positive in 87.5% (21/24) of CIN II and 69% (18/26) of CIN I, while the HR HPV was positive in 75% (18/24) of CIN II and 50% (13/26) of CIN I. In ASCUS cytology, p16INK4a and HR HPV showed the same rate of positivity (28.5%; 2/7). Expression of p16INK4a was detected in all cytologic (10/10) ASC-H lesions, in contrast to HR HPV detected in only 20% (2/10) of ASC-H cases. These data suggest the p16INK4a evaluation in ThinPrep cervical samples to be significantly associated with HR HPV testing by PCR in the same sample for the diagnosis of HSIL lesions and cervical carcinomas. A prospective study with longer follow up may clarify the predictive values in the management of LSIL and ASC diagnosis